The requirements for the enhancement of transmigration in vitro also apply in vivo.

Fluorescently labeled CD34⁺ cells mixed with CD8⁺ cells that had been pretreated with brefeldin A or cytochalasin D were injected intravenously. Homing to the bone marrow was evaluated by flow cytometry. The solid bars represent mean values. Each data point represents results from an individual mouse. Experiments using cells from the same donor source are linked by dashed lines.