Cytospin and Northern blot analysis of the neutrophil cell populations before and after MACS purification. (A) Cytospin preparations of cells from the 3 Percoll-separated bone marrow populations (MBs/PMs, MCs/MMs, and BCs/SCs) and peripheral blood granulocytes (PMNs) before and after MACS depletion of nonneutrophil cells. Cells were stained with May-Grünwald-Giemsa. (B) Cytospins of granulocytic populations from peripheral blood stained with May-Grünwald-Giemsa (MGG) and Fast Green/Nuclear Red. (Top) Total granulocytes isolated from peripheral blood by means of Lymphoprep. Neutrophils are dominant, but eosinophils are visible (characterized by their bilobed nucleus, intense red granules in MGG staining, and green granules in Fast Green staining,⁸⁴  respectively). (Middle) Total granulocytes depleted for CD49d-expressing cells. (Bottom) The CD49d⁺ fraction from total granulocytes highly enriched in eosinophils. (C) Northern blot of RNA from the 3 granulocytic populations. The blot was hybridized to C/EBP-ϵ, CLC, and 18S.