ND13 expression in BM confers a growth advantage to transduced BM cells. (A) Retroviral vectors used to express ND13 and Meis1 in murine BM. The FG repeats of the NUP98 gene and the homeodomain of HOXD13 are illustrated as hatched boxes and black boxes, respectively. (B) Western blot analysis of nuclear extracts from 293T cells transfected with FLAG-ND13 and HA-tagged Meis1 as detected with an anti-FLAG and an anti-HA monoclonal antibody, respectively. Molecular mass markers are indicated. (C) Cell expansion of transduced (GFP⁺) and nontransduced (GFP^-) cells in cultures initiated with BM infected with GFP control or ND13 virus (mean ± SD). Results of a representative experiment (n = 3) are shown; each experiment was performed in triplicate cultures. (D) Wright-Giemsa (original magnification, × 400) staining of a representative ND13-transduced, IL-3–dependent cell line that underwent monocytic differentiation with M-CSF stimulation but remained predominantly blastic without granulocytic differentiation with G-CSF stimulation. ND13 indicates NUP98-HOXD13; FS, fusion site; LTR, long terminal repeat; and CTL, control.