Chemokine-triggered transendothelial migration of patient PBLs is partially defective, but chemotaxis is conserved. (A) Migration of PBLs accumulated on TNF-activated HUVECs alone or overlaid with SDF-1 (100 ng/mL), subjected to physiological shear stress (5 dyne/cm2) for 20 minutes. The 4 indicated categories of lymphocytes accumulated on the HUVEC monolayer were defined as outlined in “Materials and methods.” White bars indicate detachment; light gray bars, arrest; dark gray bars, locomotion; black bars, TEM. Results represent the mean ± SEM of 3 independent experiments. (B) SDF-1–triggered PBL chemotaxis determined in a transwell filter assay. Control or LAD PBLs were allowed to migrate toward the indicated concentrations of SDF-1 placed in the lower chamber of a BSA-coated transwell. Light gray bars indicate no SDF-1; dark gray bars, 10 nM SDF-1; black bars, 50 nM SDF-1. Results are average ± range of duplicate wells.
