Association of the γ chain with transfected WT hFcγRI but not with the hFcγRI chimera hFcγRI-CD8-hFcγRI (EC-TM-CY). COS-1 cells were cotransfected with His-tagged human γ chain and either FcγRI-CD8-FcγRI/Myc (lane 1) or FcγRI/Myc (lane 2). Sham-transfected cells are shown in lane 3. Cell lysates were immunoprecipitated with anti-Myc antibody and blotted with anti-His antibody. Both flow cytometry and Western blot demonstrated that WT hFcγRI and hFcγRI-CD8-hFcγRI were similarly expressed, and Western blot showed that WT hFcγRI and hFcγRI-CD8-hFcγRI were similarly immunoprecipitated by anti-Myc antibody.