![Fig. 6. Immunization with apoptotic 12B1-D1 cells plus CRCL adjuvant induces IL-2 and IFN-γ secretion and T-cell proliferation of splenocytes. / 12B1-D1 cells were treated with 100 μg/mL Mit-C for 1 hour and then extensively washed. Then 20 μg/mouse liver lysate, liver-derived HSP70, or CRCL was added to the cells and the mixture was injected to BALB/c mice subcutaneously on days −14 and −7. Control mice were immunized with an equal number of Mit-C–treated 12B1-D1 cells or saline. Five days after the secondary immunization, splenocytes of the immunized mice were harvested and restimulated with Mit-C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]-thymidine incorporation. (Representative data from 1 of 3 experiments are shown.)](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/1/10.1182_blood-2002-05-1580/6/h80133618006.jpeg?Expires=1735547212&Signature=tvUHE8VzqZTMIew3wUMI81yK1efD6Rvn0XpS~Zd96DfWtGLpmAut3mxDGykAKpK1ChQ8FIbe9G0OF~gu4~fGE8vPHuHmSC99nxmowLq6xzFi4Q3DFJJnJv0GV1ZYoF-TrDxNO3eYUpnlZT5MZLrwms-M8a72rV1B6LCZx9e4IXCIcpnlISYKFicrFub~ozC0a8KjgTCN6aRTN5w4N~-3eTm52YHGSAVZaZuX3eYLtBbTJI179y1UYnsm8MHIfCnFAS2~7QXCHZhLS9gIFF8Uj6wO-5bORxJ6~c2l-tpqlqg9GPSo3tN35QAzQes28-kTnQRzLNr4t6Yr6uc5ExyBTA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunization with apoptotic 12B1-D1 cells plus CRCL adjuvant induces IL-2 and IFN-γ secretion and T-cell proliferation of splenocytes.
12B1-D1 cells were treated with 100 μg/mL Mit-C for 1 hour and then extensively washed. Then 20 μg/mouse liver lysate, liver-derived HSP70, or CRCL was added to the cells and the mixture was injected to BALB/c mice subcutaneously on days −14 and −7. Control mice were immunized with an equal number of Mit-C–treated 12B1-D1 cells or saline. Five days after the secondary immunization, splenocytes of the immunized mice were harvested and restimulated with Mit-C–treated 12B1-D1 cells. (A) CTLL-2 bioassay was used to determine the IL-2 production. (B) IFN-γ secretion was determined by ELISPOT. (C) T-cell proliferation was determined by [3H]-thymidine incorporation. (Representative data from 1 of 3 experiments are shown.)
