Peripheral blood analysis of mice reconstituted with control GFP- or Pax5-expressing cells. (A) Leukocytes were gated by forward and side scatter and also for the Ly-5.2 donor cell marker. Histograms indicate the percentage of GFP⁺ donor cells from representative animals. (B) The proportion of GFP⁺ donor cells in the peripheral blood decreases with time in animals reconstituted with Pax5-expressing cells. Each time point is representative of 5 control GFP- and 7 Pax5-reconstituted animals (P < .01). (C) Donor cells (Ly-5.2⁺) were gated and then analyzed for the percentage of B220⁺ cells in the peripheral blood. Representative plots are shown. (D) Proportion of B lymphocytes among the donor-derived GFP⁺ cells in the peripheral blood. The data are representative of 5 control GFP- (□) and 7 Pax5-reconstituted (▪) animals for each time point analyzed (P < .01 at 3, 6, and 18 weeks). (E-F) The percentages of peripheral blood GFP⁺ cells that stain positive for CD3, Gr-1, and Mac-1 are indicated. Data are representative of 4 control GFP- and 7 Pax5-reconstituted animals analyzed at 18 weeks after transplantation.