Analysis of lymphocyte development in spleen and thymus. (A-B) κ/λ light chain expression in peripheral blood of animals reconstituted for 12 weeks. The data are representative of 5 control GFP- and 7 Pax5-reconstituted animals. Percentages of cells within the gated quadrants are shown. (C) The percentage of B220⁺ B cells in spleen was analyzed in 3 control GFP- and 3 Pax5 animals at 12 weeks after transplantation. (D) The percentage of Pax5⁺GFP⁺ B lymphocytes in the spleens of reconstituted animals was statistically significant (P < .01). (E-F) Absence of Pax5-expressing thymocytes. The data are representative of 6 control GFP- and 6 Pax5-reconstituted animals analyzed at 20 weeks after transplantation.