Bone marrow analysis of control GFP- and Pax5-expressing cells. (A) Donor-derived bone marrow cells from representative animals were stained for B220, Gr-1, Mac-1, and NK1.1. Percentages of cells within the gated quadrants are shown. (B) Percentages of donor-derived GFP⁺ cells that express B220 (P < .10), Gr-1 (P < .22), Mac-1 (P < .24), and NK1.1. (P < .50). Data are representative of 3 control GFP- and 3 Pax5-reconstituted animals receiving transplants 12 weeks previously. (C-D) NK1.1 staining of spleen samples from animals receiving transplants 12 weeks before (n = 3 for both constructs, P > 0.7). The cells (histograms) shown are gated on viable, GFP⁺ splenocytes based on propidium iodide staining.