Figure 4.
![Figure 4. Immunization with DCs pulsed with 12B1-derived CRCL provides significant protection to 12B1 tumor challenge. DCs were incubated with 50 μg/mL 12B1-derived CRCL or the same concentration of purified HSP70 or unfractionated lysate protein for 24 hours. DCs (5 × 105) were injected subcutaneously into the groin on days - 14 and - 7, and the mice were challenged with 12B1 cells (103) in the opposite groin on day 0 (saline versus DC, not significant [ns]; DC versus DC + lysate, ns; DC versus DC + HSP70, P < .05; DC versus DC + CRCL, P < .0001; DC + HSP70 versus DC + CRCL, P < .005; pooled data from 2 experiments, n = 12-16 mice per group).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/11/10.1182_blood-2002-10-3108/5/h81134417004.jpeg?Expires=1735547123&Signature=15J22yPKB6-HzSvY0LVzvZyFN4hEHb9Cv3r0Jd1a4-bdnld-6iPGxVkB9HOTDxkpOqUbrI546X-EbKh8Nl5yABa0glxtb8nt4tRwx2YAtlaOQ2jOd5reP2BHPIRlHsRqBRWWg6vaT5omYTXKv3~CtJnPSo9Fq8~86rV9k1ou8BfNH7yXshndENrFFWESwKnLpmqW9GCF6HA82GgZUY82Is3WvgW0RfKb0MMnyRrqDoPDAsOrCsQW-1q9zJVz6pWducSvdETDhdrkXDtKItzBzJLyeFkPFGPafp4X-rRq6H5DjVSPvGyL327BvYbrgIDbt9Or2~tdVEo278chycoz7A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunization with DCs pulsed with 12B1-derived CRCL provides significant protection to 12B1 tumor challenge. DCs were incubated with 50 μg/mL 12B1-derived CRCL or the same concentration of purified HSP70 or unfractionated lysate protein for 24 hours. DCs (5 × 105) were injected subcutaneously into the groin on days - 14 and - 7, and the mice were challenged with 12B1 cells (103) in the opposite groin on day 0 (saline versus DC, not significant [ns]; DC versus DC + lysate, ns; DC versus DC + HSP70, P < .05; DC versus DC + CRCL, P < .0001; DC + HSP70 versus DC + CRCL, P < .005; pooled data from 2 experiments, n = 12-16 mice per group).
