Antitumor immunity induced by DCs pulsed with 12B1-derived CRCL is both CD4⁺ and CD8⁺ T-cell dependent. On days - 14 and - 7, mice were immunized subcutaneously with DCs (5 × 10⁵) that had been preincubated with 50 μg/mL 12B1-derived CRCL for 24 hours. Groups of mice were injected intraperitoneally with 200 μg anti-CD4 and/or anti-CD8 antibodies on days - 3, - 1, + 1, and + 3. On day 0, mice were challenged with 12B1 cells (10³) in the opposite groin (saline versus CD4 and CD8 depletion, ns; CRCL versus CD4 and CD8 depletion, P < .05; n = 4 mice per group, representative data from 1 of 2 experiments).