DCs pulsed with 12B1-derived CRCL are effective therapeutic vaccines in tumor-bearing mice. DCs were incubated with 50 μg/mL 12B1-derived CRCL or the same concentration of purified HSP70 for 24 hours. Mice were injected with 12B1 cells (10³) subcutaneously in the right groin on day 0 and injected with CRCL- or HSP70-loaded DCs (5 × 10⁵) in the opposite groin on day 2 (DC versus DC + HSP70, ns; DC versus DC + CRCL, P < .05; DC + HSP70 versus DC + CRCL, P < .005; pooled data from 2 experiments, n = 8-16 mice per group).