Response of freshly isolated AML cells to GO in vitro Peripheral blood mononuclear cells isolated from patients with AML were cultured in vitro in the presence of IL-3 and GM-CSF and in the presence of increasing concentrations of GO. 3H-thymidine uptake and cell viability were measured at 48 to 60 hours. The results are expressed as percentage of the untreated controls. (A) Dose-response curve of thymidine uptake of patients 4 (•), 6 (□), and 8 (▴). (B) Dose-response curve of cell viability assay of patients 4(•), 6 (□), and 8 (▴). (C) Dose-response curve of thymidine uptake of patients 3, 5, 7, and 10. (D) Dose-response curve of cell viability assay of patients 1, 2, 3, 5, 7, 9, and 10. Pt 3 is represented by ♦; pt5, ⋄; pt2, ▪; pt9, ▵; pt7, ○; pt 1, *; and pt 10, +.
