The effect of CCL19 on the endocytosis of FITC-dextran by SDDCs and splenic DCs. SDDCs were generated by culturing splenocytes with fibroblast supernatant and GM-CSF for 14 days. Splenic DCs were freshly isolated from B6 mice. SDDCs and splenic DCs were treated with LPS for 24 or 20 hours, respectively, for use as mature DCs. Mature SDDCs or splenic DCs were incubated with FITC-dextran at 1 mg/mL in the presence or absence of CCL19. (A) Time course of endocytosis; each symbol represents the mean ± SE of 3 independent experiments. (B) Amount of FITC-Dex internalized by mature splenic DCs at 5 minutes; data are representative of 3 independent experiments.