The effect of mannan on CCL19-induced endocytosis by mature DCs. BC1 cells treated with LPS for 24 hours were used as mature DCs. Mature DCs were pretreated with mannan at 3 or 10 mg/mL for 10 minutes, then incubated with FITC-dextran (Dex) at 0.2 or 1 mg/mL in the presence of CCL19. The FITC-Dex uptake was determined 2 minutes after treatment. Each column represents the mean ± SE of 3 independent experiments.