Blocking of rIE^CSA and mature IE^CSA adhesion to Sc1D cells in the presence of specific inhibitors. A culture of rIE^CSA and mature IE^CSA parasites (equal parasitemia) was passed over confluent Sc1D cells in microslides at 0.05 Pa. (A) Sc1D cells with bound rIE^CSA and mature IE^CSA. (B) Flushing with 2 mL 100 μg/mL anti–RSP-2 mAb B4 in culture medium. (C) Flushing with 2 mL 100 μg/mL CSA dissolved in culture medium. (D) nE, rIE^CSA, and mature IE^CSA were desequestrated using both inhibitors. Scale bar measures 10 μm.