LF 15-0195 sensitizes Jurkat T cells to TRAIL- and anti-CD3–induced cell death.

(A) Jurkat T cells were treated with either vehicle or 0.1 μM LF 15-0195 for 3 days before exposure to indicated concentrations of TRAIL or TNFα (without or with 0.8 μg/mL cycloheximide). Apoptosis was studied by Hoechst 33342 staining of the nuclear chromatin. ■ represents vehicle alone; ▪, LF 15-0195. (B) Wild-type (WT), FADD^−/−, and caspase-8^−/− Jurkat T cells were treated with either vehicle (■) or 0.1 μM LF 15-0195 (▪) for 3 days, then treated with an anti-CD3 Ab for 24 hours. Apoptosis was studied by Hoechst 33342 staining of the nuclear chromatin.