LF 15-0195 sensitizes human peripheral T cells to AICD.

Peripheral T cells were purified from blood samples from healthy volunteers. (A) Cells were exposed to OKT3 Ab for 24 hours, then cultured in the presence of IL-2 for indicated times. Every day, an aliquot of cells was exposed to CH11 Ab (25 ng/mL) for 6 hours, and apoptosis was determined by Hoechst 33342 staining. (B) Cells were exposed to OKT3 Ab for 24 hours, then cultured in the presence of IL-2, then restimulated with OKT3 Ab for 24 hours at day 5 (d5-6) or 48 hours at days 3 (d3-5) or 5 (d5-7) before studying apoptosis as described in the legend to Figure 1. (C) Cells were exposed to OKT3 Ab for 24 hours, then cultured in the absence or presence of IL-2, in the presence or absence of an anti-CD95L Ab (Nok1, 5 μg/mL) for indicated times. Then, apoptosis was studied as in Figure 1. Results are expressed as means ± SD of triplicate experiments. One representative experiment is shown. ■ represents cells cultured with vehicle alone; ▪, cells cultured in presence of 0.1 μM LF 15-0195.