Arrest of flow platelets incubated with or without a blocking anti-αIIbβ3 mAb on immobilized peptides 4N1K ± ICAM-1. Permanox Lab-Tek 1 chamber slides were coated with 100 μM 4N1K ± 30 μg rhICAM-1 peptides (see “Materials and methods”) and were placed in the flow chamber. Platelets were first labeled with calceine in PRP and then were incubated or not incubated with 40 μg/mL anti-αIIbβ3 mAb P2 for 10 minutes at 37°C. Whole blood was reconstituted and perfused through the chamber at 37°C and at a shear rate of 100 seconds–1 for 3 minutes. Platelet adhesion, expressed as percentage of surface covered with platelets, is the average ± SEM of 10 random fields per coverslip. ***P < .001. This experiment is representative of 3 experiments performed using blood from different donors.
