Figure 3.
![Figure 3. The effect of low extracellular [Ca2+]i (50 nM) and thapsigargin on the APC-induced [Ca2+]i signal. (A) BECs in media containing 1.8 mM or 50 nM Ca2+ were subjected to APC (100 nM) perfusions. Traces represent [Ca2+]i of 12 to 15 single cells from a representative experiment. Horizontal line indicates the time of APC addition. (B) Thapsigargin (1 μM; TG) was introduced to BEC culture as indicated by the horizontal line, and APC (100 nM) was administered for 60 seconds at the time indicated by the arrow. Traces show [Ca2+]i in single cells from representative experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-12-3680/6/h81234484003.jpeg?Expires=1735550852&Signature=X4-Uof~Qws5ED9nJTmd7B43p-YijeJYoL3MVIAWza1p~p0mNZxW1WYcsz2Dq7SDHnwupPv88Ow1I34A8wxf0OMIchMSXAlUZG2b~jfeOK4akyXBmZwL0BfguMzUyrRxO5FqdDqrgwpsWXiLvQCmrhxgJabb~PuLKMH3vf740M4aT-9l8-boispIGrPJwMdr0TRn4vEgRf2ztJWV6vCI1iu3BghopNer1vUHmmCm2sn0P5XVe9LxNluhRaX2l40duYnzb9AsU5z4g~brgvMXiSn2LigQrm8l8J9im5CenlhEWcCii-sWM57ceVfJTQTPVFBfgWTJSR8wWLejDjjS7iA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The effect of low extracellular [Ca2+]i (50 nM) and thapsigargin on the APC-induced [Ca2+]i signal. (A) BECs in media containing 1.8 mM or 50 nM Ca2+ were subjected to APC (100 nM) perfusions. Traces represent [Ca2+]i of 12 to 15 single cells from a representative experiment. Horizontal line indicates the time of APC addition. (B) Thapsigargin (1 μM; TG) was introduced to BEC culture as indicated by the horizontal line, and APC (100 nM) was administered for 60 seconds at the time indicated by the arrow. Traces show [Ca2+]i in single cells from representative experiments.
