Figure 2.
Analysis of platelet-derived TAFI. Platelet-derived TAFI and TAFI purified from plasma (lane 1; 300 pg) were analyzed on Western blot (A). Gel-filtered platelets (3 × 107 cells) were incubated with either 0.5% triton (lane 2), buffer (lane 3), or 0.5 U/mL thrombin (lane 4) 15 minutes at 37°C. Deglycosylation of platelet-derived TAFI (B). Purified TAFI (16 ng, lane 1, or 1.6 ng, lane 2) and gel-filtered platelets (7 × 107 cells) incubated with thrombin (0.5 U/mL) for 15 minutes at 37°C (lane 3) were subjected to PNGase F treatment. TAFI was detected with a monoclonal antibody against TAFI (9H10). Approximate molecular masses (kDa) are shown on the left.
