Cytoskeletal reorganization and chemotaxis in response to SDF-1 of cells from normal or Ptx-treated mice. Mobilized lineage-depleted cells from Ptx-treated mice did not display SDF-1–induced actin polymerization after 10 seconds (top right panel, PB, dark columns) compared to control cells (white columns). BM cells from treated mice were also modestly affected (top left panel, BM, dark columns). Furthermore, when Lin^-/- cells from BM or PB were used for migration assays (bottom panels), cells from Ptx-treated mice (dark columns) were largely SDF-1 unresponsive.