cDNA constructs used for mapping the NoS in RPS19. (A) Human wild-type RPS19 (H WT RPS19) is shown at the top of the panel as the black box. The ATG translational start site and the last codon coding for a histidine (H) are indicated. We generated 6 COOH-terminal truncations. The mutant GFP-RPS19 fusion proteins are labeled by the last amino acid number in the new COOH-terminal and by the number of deleted amino acids (Δ). We added a stop codon (located with arrow-heads) just before a putative NLS characterized by a cluster of basic amino acids: arginine (R), histidine (H), lysine (K). (B) We also generated 7 NH₂-terminal truncations in GFP-RPS19 fusion proteins. These are labeled with the amino acid number of the NH₂ terminus and by the number of deleted amino acids (Δ). We used EGFP ATG as the translational start site of the GFP-RPS19 fusion proteins, meaning that RPS19 was cloned in frame with upstream EGFP coding sequence.