Gp75 expression on B16F10 melanoma cells.

The interaction of mAb TA99, which binds the gp75 melanocyte differentiation antigen, with in vitro–grown B16F10 cells was analyzed by flow cytometry. B16F10 cells were incubated with control mIgG2a (thin solid line) or TA99 (thick solid line), and FITC-conjugated anti–mouse IgG, to assess gp75 membrane expression. Total gp75 expression was assayed by TA99 staining on permeabilized B16F10 cells (dashed line).