HIF-1–dependent reporter gene activation by oxLDL. MM6 cells were transiently transfected with a luciferase reporter gene construct, containing (A) 3 copies of the HRE of the erythropoietin gene (pGL3-EPO-HRE-SV40-LUC) or (B) pGL3-1.5kbVEGFprom. Transfected cells were stimulated for 8 hours with 200 μg/mL oxLDL or nLDL, in the absence or presence of 30 μM DPI, or exposed for 8 hours to hypoxia (1% oxygen) followed by luciferase activity determination. Luciferase activity is expressed relative to controls set as 100%. Values represent the fold induction of 4 independent experiments.