Figure 5.
Figure 5. Promoter activity of the human IL-12(p35) gene and 5′ deletion analysis of the promoter region. A series of luciferase (LUC) reporter constructs (□) were generated using the pGL3basic vector and different 5′-flanking fragments of the p35 gene. Positions of the 5′ ends of the various fragments and the region protected by nuc-2 are indicated. RAW 264.7 cells were transiently transfected with 2 μg of the reporter plasmids and 20 ng pRL-TK as an internal control plasmid. RAW 264.7 cells were then left untreated (□) or treated with IFN-γ and LPS (▦). Luciferase activities (Firefly and Renilla) were measured in cellular extracts. Promoter activities were normalized using Renilla luciferase activities. Each point is the mean ± SD of triplicate transfections performed in the same experiment. A representative experiment of 3 independent transfections is shown.

Promoter activity of the human IL-12(p35) gene and 5′ deletion analysis of the promoter region. A series of luciferase (LUC) reporter constructs (□) were generated using the pGL3basic vector and different 5′-flanking fragments of the p35 gene. Positions of the 5′ ends of the various fragments and the region protected by nuc-2 are indicated. RAW 264.7 cells were transiently transfected with 2 μg of the reporter plasmids and 20 ng pRL-TK as an internal control plasmid. RAW 264.7 cells were then left untreated (□) or treated with IFN-γ and LPS (▦). Luciferase activities (Firefly and Renilla) were measured in cellular extracts. Promoter activities were normalized using Renilla luciferase activities. Each point is the mean ± SD of triplicate transfections performed in the same experiment. A representative experiment of 3 independent transfections is shown.

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