RT-PCR analysis of CTLA-4 transcripts from normal and neoplastic, resting and activated, hematopoietic cells.

Total RNA from the indicated cells was reverse transcribed and PCR amplified with primers specific for CTLA-4 full-length coding sequence (A) and for CTLA-4 extracellular domain (B). Nested PCR was performed on the first-round CTLA-4 full-length PCR product as template with CTLA-4 extracellular domain inner primers (C). As internal control, β-actin gene amplification was carried out (D). PBMCs are freshly isolated peripheral blood mononuclear cells; Raji and Jurkat are B- and T-lymphoblastic cell lines, respectively; and AML is an adult acute myeloid leukemia sample. Molecular weights are expressed as base pairs (bp).