Western blot analysis of CTLA-4 protein from resting and activated B-lymphoblastic (Raji) and T-lymphoblastic (Jurkat) cell lines.

Proteins from cell lysates were subjected to SDS–polyacrylamide gel electrophoresis (SDS-PAGE) on a nonreducing 12% (wt/vol) polyacrylamide gel, transferred to nitrocellulose membrane, and probed with scFv 83 mixed to the anti–c-myc tag 9E10 mAb. The reaction was visualized by ECL.