SDS-PAGE analysis of chTNT-3/IL-2 WT and IL-2 analog fusion proteins. Coomassie blue-stained 10% polyacrylamide tris-glycine reduced gel of purified chTNT-3 (lane 1), chTNT-3/IL-2 (lane 2), chTNT-3/Asp20Lys (lane 3), chTNT-3/Arg38Gly (lane 4), chTNT-3/Arg38Trp (lane 5), chTNT-3/Met39Val (lane 6), chTNT-3/Met39Leu (lane 7), chTNT-3/Phe42Lys (lane 8), chTNT-3/His55Tyr (lane 9), and molecular weight markers (lane 10).