Figure 8.
A PAR domain protein enhances promoter activity through the PAR motif from the LMO2 gene. (A) The 30-mer oligonucleotides representing the wild-type and mutated PAR domains. The consensus PAR-binding site is underlined; the mutated nucleotides are shown in bold with an asterisk. (B) Schematic diagram of the minimal promoter constructs containing 3 copies of the oligomers. (C) Analysis of the reporter gene activity in transient cotransfection experiments in HEK 293 cells.