Figure 2.
Figure 2. Expression of lentiviral vector sets in pooled populations of KG1a cells. (A) FACS histograms of GFP fluorescence 4 months after transduction with the MU3 promoter–containing set of lentiviral vectors. (B) FACS histograms of GFP fluorescence 4 months after transduction with the EF1α promoter–containing set of lentiviral vectors. (C) FACS histograms of GFP fluorescence 4 months after transduction with the CAG promoter–containing set of lentiviral vectors. Shown in each case are the MFI values of fluorescence intensity and the CV values indicative of fluorescence homogeneity. To the right of each series of histograms are the Southern blot analyses of BamHI-digested genomic DNAs hybridized with a GFP probe (top panels) and a bcl-2 probe (bottom panels) showing comparable proviral vector copy numbers per cell.

Expression of lentiviral vector sets in pooled populations of KG1a cells. (A) FACS histograms of GFP fluorescence 4 months after transduction with the MU3 promoter–containing set of lentiviral vectors. (B) FACS histograms of GFP fluorescence 4 months after transduction with the EF1α promoter–containing set of lentiviral vectors. (C) FACS histograms of GFP fluorescence 4 months after transduction with the CAG promoter–containing set of lentiviral vectors. Shown in each case are the MFI values of fluorescence intensity and the CV values indicative of fluorescence homogeneity. To the right of each series of histograms are the Southern blot analyses of BamHI-digested genomic DNAs hybridized with a GFP probe (top panels) and a bcl-2 probe (bottom panels) showing comparable proviral vector copy numbers per cell.

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