IL-10–/–CD4+ T cells become tolerant and develop in vitro regulatory function during MLR culture with IL-10 and TGF-β (A) IL-10–/–CD4+ cells become tolerant after MLR culture with IL-10 and TGF-β. MLR consisted of B6 IL-10–/–CD4+ responders (0.5 × 105/mL) and bm12 splenic stimulators (0.5 × 105/mL) cultured with and without IL-10 and TGF-β for 8 days. Eight-day MLR cultured B6 IL-10–/–CD4+ were washed free of cytokines and restimulated with bm12 splenic stimulators. Shown is 1 of 3 experiments with similar results. (B) IL-10/TGF-β–treated IL-10–/–CD4+ T cells inhibit naive alloresponses in vitro. 105 naive B6 IL-10–/–CD4+ T-cell responders per well and 105 bm12 splenic stimulators per well were plated alone or with 2.5 × 104 or 105 8-day B6 IL-10–/–CD4+ cells that had been previously tolerized to bm12 alloantigen by an 8-day culture with IL-10 and TGF-β. Shown is 1 of 3 representative experiments.
