Injection of αDEC conjugates inhibits T-cell proliferation in DTH reactions in vivo. (A) Mice were treated as described in the scheme. (B) After challenge with 30 μg OVA peptide in IFA into the right footpad, footpad thickness was measured after 24 hours using a caliper rule. The asterisk indicates a significant difference (mean of 5 mice/group; α < 0.05) from antibody control (G1117-OVA). Results from 1 of 3 typical experiments are shown. (C) LN cells obtained from mice described in panel B were prepared and cultured in the presence of OVA, as indicated. ▴ indicates GI117-OVA; •, α MHCII-OVA; ▪, αDEC-OVA + αCD40; and ○, αDEC-OVA. T-cell proliferation was assayed by ³H-thymidine uptake.