RT-PCR analysis of c-met, VE-cadherin, and β-actin mRNA in HUVECs grown in different culture conditions. Total RNA extracted from confluent and sparse HUVECs and from the HUVECs grown in 3-dimensional collagen gel was reverse transcribed with AMV reverse transcriptase and amplified using PCR, as described in “Materials and methods.” Amplified products were analyzed on a 1.5% agarose gel after staining with ethidium bromide. Figures on the top indicate the dilution of the single-stranded cDNA template used for the PCR. Results are representative of 4 independent experiments.