Figure 2.
![Figure 2. Effect of S1P on polyclonal T-cell proliferation. T cells were incubated with 10 ng/mL PMA plus ionomycin (IONO), or 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28, in the absence or the presence of 0.1 to 10 μM S1P for 3 days at 37°C. At this time, 1 μCi (0.037 MBq) [3H]thymidine ([3H]Tdr) was added to each well, and the cultures were incubated for an additional 6 hours before harvesting (A). The same experiment was conducted in serum-free media (SFM) (B). T cells (1 × 106/mL) were treated with 100 ng/mL PTX for 18 hours. These cells were washed 3 times, and then incubated with the various stimuli in the absence or presence of different concentrations of S1P (C). cpm indicates counts per minute.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-09-2962/6/h81234468002.jpeg?Expires=1735550496&Signature=TE1QWLhpIMdsH62t-j~WdPaf40SgeNp5-snsgYTVeHVB6zju6gJaAbuD2N5tBvkpljeQREw4nfgq690VLvC9acvUDGwbXbXr-zSZfEnD7GaDDFc2uPVV3ZVAfiR2J26Mwq~tt3kQssNSM7JLi-nH0N7qTcVNTT8n7nb8~mxe~xsbvJ6BWG0QZiApZWze1BuM7S5UHRi-O5kgerF22QBBXuQsRHZXDDgXOA-BM9ce4O8S12AXwNyCRKoT9pDWEYgwbCCt~BWYAmqpLl3VMorr~LmEGRr8-cFqGJowHuerLlTa8NN20c4r~~FrXeH94WMc-xUdsnO~EEfQCamncn~XRA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of S1P on polyclonal T-cell proliferation. T cells were incubated with 10 ng/mL PMA plus ionomycin (IONO), or 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28, in the absence or the presence of 0.1 to 10 μM S1P for 3 days at 37°C. At this time, 1 μCi (0.037 MBq) [3H]thymidine ([3H]Tdr) was added to each well, and the cultures were incubated for an additional 6 hours before harvesting (A). The same experiment was conducted in serum-free media (SFM) (B). T cells (1 × 106/mL) were treated with 100 ng/mL PTX for 18 hours. These cells were washed 3 times, and then incubated with the various stimuli in the absence or presence of different concentrations of S1P (C). cpm indicates counts per minute.
