Figure 3.
![Figure 3. Sphingolipids inhibit T-cell proliferation with different dose responses. T cells were either left intact (□, left column) or were stimulated with 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28 (▪), for 3 days in the presence or absence of different concentrations of S1P, DHS1P, ceramide (CER), or sphingosine (SPH), in media supplemented with 10% serum. The incorporation of [3H]thymidine was done as in Figure 2. P values comparing T-cell proliferation in the presence of sphingolipids to the proliferation in their absence are placed on top of each column. Data are representative of 3 experiments performed. Error bars indicate means ± SD; NS, not significant.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/101/12/10.1182_blood-2002-09-2962/6/h81234468003.jpeg?Expires=1735551901&Signature=s0eIocOXpWshwGpdJKqmtYqdbwXZlyMGivcYlKHhp7CMRJ-3CS3wBYKpBh7y4sNes2Xr5-EzRTAowX0TkcfalDwuTFXLbEHbvBu3FdP8tj6TrbxgHV5w1NCVrGsfvpmB0zgdnIrrZAlHsuC9d-Q2rLqKmTk53A7Tm0if65XLuFqabvZt1PRMWSigzIFiTr9poTTS5z-BGdSBOjg-kPzVKcJnyaHnCeiHpX9C2a60WaDkkEREd3QdxWu6oO1awmpdHbmofyUqhcMttCcP6~35NJRF5UEloKqGlG8OKJ2mBUXPGmy0JExldy2e8kg-sK9iCPxninQidL8eT3wsL-yi5g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Sphingolipids inhibit T-cell proliferation with different dose responses. T cells were either left intact (□, left column) or were stimulated with 1 μg/mL plated anti-CD3 plus 1 μg/mL anti-CD28 (▪), for 3 days in the presence or absence of different concentrations of S1P, DHS1P, ceramide (CER), or sphingosine (SPH), in media supplemented with 10% serum. The incorporation of [3H]thymidine was done as in Figure 2. P values comparing T-cell proliferation in the presence of sphingolipids to the proliferation in their absence are placed on top of each column. Data are representative of 3 experiments performed. Error bars indicate means ± SD; NS, not significant.
