Eosinophil apoptosis induced by Siglec-8 cross-linking is inhibited by the pancaspase antagonist zVAD-FMK. Cells were cultured for 24 hours, then analyzed for annexin-V staining as in Figure 3. Data are mean ± SEM from 4 experiments. *P ≤ .05 for comparison of 2E2 + 2° Ab (▨) versus 2E2 + 2° Ab + zVAD-FMK (▦). ▪ indicates medium alone; □, medium + zVAD-FMK.