2ME2 alters morphology, adhesion, and motility of cells transformed with tyrosine kinase oncogenes. (A) The motility of individual cells was determined by time-lapsed video microscopy (original magnification, × 20). Ba/F3 cells transformed with BCR-ABL were either pretreated for 150 minutes with the solvent DMSO (control) or with 5 μM 2ME2. Cells were then monitored for 45 minutes and typical cells were marked by lowercase (control) or capital (2ME2) letters at the beginning (A-E; a-e) and the end (A′-E′; a′-e′) of the time period. The arrows indicate the centroid movement of typical cells (bottom panels). (B) The cell size of BCR-ABL—transformed Ba/F3 cells was determined before (control, left panel) or after 2ME2 treatment (2ME2, right panel) using a Coulter particle counter. Dashed line indicates peak position of the control. (Ci-ii) Ba/F3 cells transformed with BCR-ABL, TEL-ABL, TEL-JAK2, or TEL-PDGFβR were treated either with the solvent DMSO (▪) or with 5 μM 2ME2 (□). The adhesion of calcein-labeled cells to fibronectin (i) or transwell migration (ii) was measured after a 4-hour pretreatment with DMSO or 2ME2. The data are presented as percentage of DMSO-treated cells (n = 4). (iii) CML cells were pretreated for 2 hours either with 20 μME2(▪) or with 20 μM 2ME2 (□), and the transwell migration was measured after 1 hour. The data are presented individually as percentage of E2-treated cells (n = 2) as well as the average of 4 independent experiments. Values represent means ± SEM. *Significant differences (P < .05) were observed between 2ME2 and control-treated cells.