Dual modality imaging. (A) Dual modality imaging (γ-image, x-ray image, and merged image) at one minute following adoptive transfer of 5 × 10^{6 111}In-labeled D4 cells intravenously is shown. White box in the merged image indicates the defined region of interest used for quantitation of radioactivity in the lungs. In the red-green-blue merged image, the red channel indicates counts from the γ-image. (B) Dual modality imaging of ¹¹¹In-labeled D4 cells without (top row) or with (bottom row) anti–LFA-1 antibody after transfer intravenously at the indicated time points. Data are representative of 3 independent experiments. (C) Distribution of ¹¹¹In-labeled D4 cells at the indicated time points after transfer. Data pooled from 3 independent experiments are shown. Data are represented as percentage radioactivity (mean ± SD) in the region of interest defined for the lungs using the rib cage as a reference, with the activity at one minute represented as 100%.