Phenotype of CD4⁺and CD8⁺T cellsobtained from the blood of patients with acute MSand of healthy controls. (A) Percentage of CD4⁺ and CD8⁺ T cells before and after isolation from PBL using MACS MicroBeads. (B) Cells were phenotyped using 7-color FACS. Gates were set on either CD4⁺ T cells or CD8⁺ cells. Results shown are from 1 of 8 representative experiments. In histograms, dotted lines indicate isotype controls.