American Society of Hematology

Figure 2.

$Figure 2. CD4+ lymphocytes from patients with acute MS do not show increased rolling and sticking in brain venules. CD4+ cells were isolated from 4 patients and 4 healthy controls. Experiments were performed in 4 mice. Ten venules were examined. (A) Rolling and arrest fractions were compared between healthy controls (□) and MS patients (▪). Mean ± SD are presented. D indicates diameter. (B) Velocity histograms were generated by measuring rolling velocities. Frequency distributions were calculated after cells were assigned to velocity classes from more than 0 μm/s to 10 μm/s, 10 to 20 μm/s, 20 to 30 μm/s, and so on. Sixty-one rolling cells were examined from healthy donors (□), and 69 rolling cells were considered from patients with acute MS (▪). (C) Adherent CD4+ T cells obtained from a healthy donor (Healthy) (CMFDA-labeled) and a patient with acute MS (MS) (CMTMR-labeled) in the same cerebral venule.$

CD4⁺lymphocytes from patients with acute MS do not show increased rolling and sticking in brain venules. CD4⁺ cells were isolated from 4 patients and 4 healthy controls. Experiments were performed in 4 mice. Ten venules were examined. (A) Rolling and arrest fractions were compared between healthy controls (□) and MS patients (▪). Mean ± SD are presented. D indicates diameter. (B) Velocity histograms were generated by measuring rolling velocities. Frequency distributions were calculated after cells were assigned to velocity classes from more than 0 μm/s to 10 μm/s, 10 to 20 μm/s, 20 to 30 μm/s, and so on. Sixty-one rolling cells were examined from healthy donors (□), and 69 rolling cells were considered from patients with acute MS (▪). (C) Adherent CD4⁺ T cells obtained from a healthy donor (Healthy) (CMFDA-labeled) and a patient with acute MS (MS) (CMTMR-labeled) in the same cerebral venule.

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