HY^DbUty tetramer analysis of peripheral T cells from naive and multiparous mice. Filled squares (▪) indicate ex vivo; open squares (□), following in vitro restimulation with syngeneic male cells. Splenocytes were from naive B6, multiparous B6.EiJ, C3H.SW/SnJ, B6.HLA-A2, HW80, and B6.EiJ females mated with C3H.SW/SnJ males. In addition, spleen cells from a group of skin-grafted B6.HLA-A2 were stimulated in vitro for 7 days and analyzed for HY^DbUty-specific T cells (▵). In each case the percentage of HY^DbUty CD8⁺ T cells was assessed using MHC class I tetramers. Bars indicate means.