Release of very-high-molecular-weight VWF multimers by DDAVP but not rhIL-11. VWF multimers were separated on agarose gels and detected by immunostaining. Pooled plasma is shown for normal and homozygous VWD dogs in lanes NCP and VWD, respectively. The remaining lanes show samples drawn from C80, 1 of 2 normal dogs treated daily for 7 days with either rhIL-11 (50 μg/kg subcutaneously; lanes marked A), or DDAVP (5 μg/kg intravenously; lanes marked B). The plasma samples include baseline (BL), and days 1 and 7 at 30 minutes following treatment. Day 14 represents samples drawn 7 days after cessation of treatment. The relative multimer weights are indicated to the left of the gel: L, M, H, and VH indicate low, medium, high, and very-high-molecular-weight VWF multimers, respectively. Very-high-molecular-weight ratio (Mr) multimers are seen on day 1 with DDAVP treatment.
