Steady-state MCT-1 protein levels in exponentially growing T-lymphoid cell lines. Forty micrograms total proteins from IL-2—dependent cell lines PBL, EC155, NII85, and N1186, and IL-2—independent cell lines C10MJ, MT-2, and Jurkat were subjected to SDS-PAGE analysis and blotted on the membrane. Western blotting was performed with MCT-1 polyclonal antibody. Control immunoblotting was carried out with β-actin. All IL-2—independent T-cell lines exhibited elevated MCT-1 protein levels in contrast to the IL-2—dependent T-cell lines, including IL-2—stimulated PBLs, which have low to undetectable MCT-1 protein levels.