Confocal immunofluorescence microscopy of EC155 cell lines. EC155-Vector5 and EC155—MCT-1⁷  cells were fixed with methanol and incubated with blocking solution and primary antibody, mouse monoclonal anti-V5, or anti-PCNA. Next, secondary antimouse IgG2a-fluorescein was used for hybridization. Cells were spread on slides and mounted on coverglass with mounting medium. Slides were examined using a Zeiss LSM510 confocal scanning laser microscope. Results showed that MCT-1 overexpressing cells had elevated PCNA protein levels compared with control. Original magnification, × 40.