Induction of apoptosis by AG1296, PKC412, and SU5614 varies between cells expressing different FLT3 receptor mutants. Ba/F3 cells expressing each FLT3 mutant were incubated in the presence of (A) AG1296 (2.5, 5, 10 μM) for 72 hours, (B) PKC412 (25, 50, 100 nM) for 24 hours, and (C) SU5614 (1, 5, 10 μM) for 24 hours. Flow cytometric analysis was performed after staining the cells with Annexin V/PI. Total percentage of Annexin-V/PI-negative cells (percentage of viable cells) is shown. One representative of at least 3 independent experiments is shown.