Fanconi anemia gene transcripts are not reduced in UoC-M1. QRT-PCR reactions were performed in at least triplicate. Error bars represent standard deviations. Fold values were obtained by externally standardizing against identical amplifications in Mo7e and by internally standardizing against 18S RNA in each cell line. Compared with Mo7e, UoC-M1 does not exhibit reduced levels of mRNA for FANCA, FANCC, FANCD2, FANCE, and FANCG. An approximate 2-fold reduction in FANCF was observed. This finding was attributed to monosomy 11p in UoC-M1,¹⁰  as FANCF is encoded on chromosome 11.²⁷