American Society of Hematology

Figure 4.

$Figure 4. Nuclear FA proteins are reduced in UoC-M1 cells. Immunoblots of cytoplasmic and nuclear components were probed with antisera for the FANCA, FANCC, and FANCG proteins. Compared with the human, cervical carcinoma cell line HeLa (lane 1) and the factor-dependent, myeloid cell line Mo7e (lane 2), UoC-M1 (lane 3) has a mild reduction in the levels of cytoplasmic FANCA, FANCC, and FANCG proteins (rows 1, 4, and 5, respectively). Nuclear fractions (lanes 4-6) demonstrated a reduction of FANCC in UoC-M1 (row 4, lane 6) and a nearly complete absence of nuclear FANCA (row 1, lane 6) and FANCG proteins (row 5, lane 6). Purity of nuclear fractions was confirmed by reprobing the filters with antisera against beta-tubulin (rows 2 and 6) and topoisomerase II (rows 3 and 7).$

Nuclear FA proteins are reduced in UoC-M1 cells. Immunoblots of cytoplasmic and nuclear components were probed with antisera for the FANCA, FANCC, and FANCG proteins. Compared with the human, cervical carcinoma cell line HeLa (lane 1) and the factor-dependent, myeloid cell line Mo7e (lane 2), UoC-M1 (lane 3) has a mild reduction in the levels of cytoplasmic FANCA, FANCC, and FANCG proteins (rows 1, 4, and 5, respectively). Nuclear fractions (lanes 4-6) demonstrated a reduction of FANCC in UoC-M1 (row 4, lane 6) and a nearly complete absence of nuclear FANCA (row 1, lane 6) and FANCG proteins (row 5, lane 6). Purity of nuclear fractions was confirmed by reprobing the filters with antisera against beta-tubulin (rows 2 and 6) and topoisomerase II (rows 3 and 7).

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