Differential gene expression in CD4⁺ T cells upon G-CSF treatment in vivo quantified by real-time RT-PCR. Regulation of gene expression levels of GATA-3, interleukin-4, and interferon-γ in CD4⁺ T cells of stem cell donors upon G-CSF treatment in vivo analyzed by quantitative real-time RT-PCR. The mRNA expression was quantified by applying Taqman technology (Perkin Elmer, Wellesley, MA), and data are presented as mean (bars) of relative ratios of expression levels. Performed were 2 independent experiments of a pool of 3 stem cell donors each. SEM was below 10%.