Figure 3.
Figure 3. Construction for expression of scFv28.3-HaaT in eukaryotic cells. cDNA fragments coding for variable regions of VH and VL chains of the CD28.3 antibody were assembled with a (GGGGS)4 linker. A cDNA coding for the TVAAPS hinge peptide from the IgG heavy chain was added 3′ and fused in frame with a cDNA coding for amino acids 53 to 425 of HaaT cDNA. The complex was then introduced into the pSecTag2B expression plasmid by adding an Igκ leader to the N-terminus and a stretch sequence containing c-myc and 6HIS flag plus a stop codon to the C-terminus.

Construction for expression of scFv28.3-HaaT in eukaryotic cells. cDNA fragments coding for variable regions of VH and VL chains of the CD28.3 antibody were assembled with a (GGGGS)4 linker. A cDNA coding for the TVAAPS hinge peptide from the IgG heavy chain was added 3′ and fused in frame with a cDNA coding for amino acids 53 to 425 of HaaT cDNA. The complex was then introduced into the pSecTag2B expression plasmid by adding an Igκ leader to the N-terminus and a stretch sequence containing c-myc and 6HIS flag plus a stop codon to the C-terminus.

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